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cdt1  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc cdt1
    Cdt1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cdt1+antibody/CDT1+Rabbit+mAb/bio_rxiv__64898__2026__03__13__711538-296-48-49
    Average 93 stars, based on 77 article reviews
    cdt1 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Membrane:

    Article Title: The prognostic significance of Cdc6 and Cdt1 in breast cancer
    Article Snippet: Proteins were quantified using BCA protein assay (ThermoFisher Scientific) and equal amounts of proteins were loaded onto 4–12% SDS–polyacrylamide gel electrophoresis (SDS–PAGE) gels and electrophoretically transferred to a PDVF membrane using Novex iBlot transfer stack (ThermoFisher Scientific) on a iBlot gel transfer device (ThermoFisher Scientific). .. The membrane containing the transferred protein was blocked with 5% BSA at room temperature for 1 h. Target proteins were detected by incubating the membrane at 4 °C overnight with primary anti-Cdt1 antibody (#8064 Cell Signaling Technology) (1:500) or anti-Cdc6 antibody (#3387 cell signaling technology) (1:500) and primary anti-β-actin antibody (Santa Cruz) (1:5000). .. Blot was further developed using horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000) (Santa Cruz) and detected using Immobilon Western Chemilum HRP substrate (Merck).

    Fluorescence In Situ Hybridization:

    Article Title: Cell Ploidy Modulates Nucleolar Size via Dosage of Nucleolar Organizing Regions
    Article Snippet: .. Slides were denatured at 73°C for 12 min and hybridized at 37°C in a humidified chamber for 48 h. After FISH, nucleoli were stained with an anti-nucleolin antibody (Cell Signaling, 1:1000 dilution), or anti-CDT1 antibody (Cell Signaling, 1:200 dilution) and anti-Geminin antibody (Cell Signaling, 1:800 dilution) for 1 h at room temperature, followed by a secondary antibody conjugated to Alexa Fluor 568 (Invitrogen, 1:1000 dilution) for 1 hour at room temperature ( ). .. Slides were mounted with DAPI-containing mounting medium (Vector Laboratories).

    Staining:

    Article Title: Cell Ploidy Modulates Nucleolar Size via Dosage of Nucleolar Organizing Regions
    Article Snippet: .. Slides were denatured at 73°C for 12 min and hybridized at 37°C in a humidified chamber for 48 h. After FISH, nucleoli were stained with an anti-nucleolin antibody (Cell Signaling, 1:1000 dilution), or anti-CDT1 antibody (Cell Signaling, 1:200 dilution) and anti-Geminin antibody (Cell Signaling, 1:800 dilution) for 1 h at room temperature, followed by a secondary antibody conjugated to Alexa Fluor 568 (Invitrogen, 1:1000 dilution) for 1 hour at room temperature ( ). .. Slides were mounted with DAPI-containing mounting medium (Vector Laboratories).



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